Gene functional analyses demonstrated that ANGPTL8/betatrophin was not only involved in metabolic pathways including lipid homeostasis, especially triglyceride and cholesterol metabolism, and glucose homeostasis, especially insulin resistance, but also in AMPK signaling pathway, PI3K/Akt signaling pathway, PPAR signaling pathway, mTOR signaling pathway, HIF-1 signaling pathway, autophagy, regulation of inflammatory response. This evidence concerns the gene PPARA and Insulin resistance.