The Western blotting analysis results in Figure 1(b) showed that when compared with the saline group, Notch1 in L6-S1 SDH was significantly overexpressed in CYP-induced cystitis group at the three time points, days 8, 12, and 17 after the first CYP injection (P < 0.05 for d8, P < 0.001 for d12, and P < 0.01 for d17). This evidence concerns the gene NOTCH1 and chronic cystitis.