To investigate the contributions of TSSK4 upregulation in lung fibrosis, a vector of small-hairpin RNA targeting TSSK4 was imported into C57BL/6 mice by lentivirus-mediated intrapulmonary infection, to suppress TSSK4 expression in vivo (Fig. 2G, first panel). The gene discussed is TSSK4; the disease is pulmonary fibrosis.