To further study the regulatory mechanism between FAM49B, Rab10, and TLR4 in BC, FAM49B knockdown in the BC cell lines MCF-7 and MDA-MB-231 was performed using shRNA, and we found that FAM49B knockdown significantly inhibited the protein expression of Rab10 and TLR4 in BC cell lines. The gene discussed is RAB10; the disease is breast cancer.