The mechanism of action assays for 6-shogaol in various cancer cells showed that it is involved in multiple signaling pathways during initiation, progression, and metastasis.26-29,32 As one of the important pathways in tumorigenesis, once the PI٣K/AKT/mTOR signaling pathway is activated, various oncogenes that can lead to cancer progression are upregulated.33 Yang and Wang investigated the role of AKT in oral cancer using different compounds.9,34 Kim, et al.11 (2014) reported that 6-shogaol could inhibit cell proliferation in lung cancer by directly targeting AKT. This evidence concerns the gene MTOR and lip and oral cavity carcinoma.