CEACAM5 and neoplasm: The role of multilayer molecular profiling in the discrimination of patients with larger tumors (T3-T4) and distant metastases was evaluated by including analytes such as concentration of oncoproteins CA 19-9, Cancer Antigen 72-4, CEA, MSI status, TMB, quantity of somatic mutations (unique or matching the tumor tissue), presence or absence of somatic mutations (unique or matching the tumor tissue), and specific mutations of the most mutated genes.