In this study, the suppressing effects of tRF3‐Thr‐AGT overexpression on cell pyroptosis and inflammation in AP models were abrogated by upregulating ZBP1, suggesting that tRF3‐Thr‐AGT exerted its protective effects during AP development by inhibiting ZBP1‐mediated cell pyroptosis. The gene discussed is ZBP1; the disease is alkaline phosphatase measurement.