Then, the AR42J cells were exposed to STC to induce cellular models for AP, and the cells were grouped as follows: Control, STC group, STC + OE‐tRF3‐Thr‐AGT group, STC + OE‐tRF3‐Thr‐AGT + OE‐ZBP1 group, and STC + OE‐tRF3‐Thr‐AGT + OE‐NLRP3 group. The gene discussed is NLRP3; the disease is alkaline phosphatase measurement.