CRHR1 and major depressive disorder: However, CRHR1 deletion in Mor-Ens, but not in Sal-Ens increased the entry time in the open arm (Fig. 6m and Supplementary Fig. 14f), the struggling time in tail suspension test (TST), and social-preference score during opiate withdrawal (Fig. 6o, p and Supplementary Fig. 14e, g), suggesting that CRHR1 of Mor-Ens contributes to the development of the negative effect, including anxiety, depression, and reduced sociability during opiate withdrawal.