SIRT2 and medical procedure: To assess the potential of SIRT2 to reverse alcoholic liver injury, we developed a recombinant adeno-associated viral vector serotype 8 (AAV8) expressing SIRT2 or its catalytic inactive SIRT2-H187A (H187A) mutant17,18 under the control of the hepatocyte-specific thyroxin-binding globulin (TBG) promoter (AAV8-SIRT2 and AAV8-H187A) (Supplementary Fig. S4, a).