Additionally, immunofluorescence assay showed that, besides cytomembrane, TRPC1 also located in the cytoplasm of colorectal cancer cell lines HT29, SW620, and HCT116, which highlighted the possible role of TRPC1 as the cytoplasmic protein in CRC cells (Supplementary Fig. S8D). Here, TRPC1 is linked to colorectal carcinoma.