To solidly prove that Src–matrine interaction plays a critical role in the regulation of cancer cell proliferation and phosphorylation signaling pathways, a mutant plasmid of pcDNA3.1-HA-SRC-A392G was constructed, which loses the binding ability to matrine without affecting normal kinase activity of Src (Fig. 3F and Supplementary Fig. S6). This evidence concerns the gene MMP7 and cancer.