In order to investigate whether ROS play any role in GBM cell invasion under the influence of Par3, we assessed invasiveness of U3031MG/MS and U3034MG/MS cells upon silencing of Par3 and further treatment with two antioxidants, butyl-hydroxyanisole (BHA) and N-acetyl-L-cysteine (NAC), in the transwell assay using a laminin matrix (Fig. 6). This evidence concerns the gene PARD3 and glioblastoma.