Kaplan–Meier curves of relapse-free survival times of breast cancer patients with lymph node positive status, revealed patients with high FN1 and PLAU expression (the median value in the entire patient dataset was used as the cut off for low and high expression) have reduced survival profiles compared to patients with low FN1 and PLAU expression (Fig. 5d and f). This evidence concerns the gene PLAU and breast cancer.