Microarray data analysis revealed heat maps of differentially expressed EMT genes upon flow stimulation for each breast cancer cell line, including consistent upregulation of EMT inducers such as FN1, SNAI2, SERPINE1, NOTCH1, TCF4, MMP2, PLAU, WNT5A and WNT5B in most flow stimulated cells (Fig. 2a, c, e and g). This evidence concerns the gene TCF4 and breast cancer.