Furthermore, we showed that targets of licorice active compounds correlated with CD8+ T-cell infiltration in TCGA LUAD patients . And the immune phenotypes of TCGA LUAD patients were evaluated by Thorsson et al. [40] , (Fig. 6a, Additional file 1: Figs. S4b, c). Then intratumoral CD8+ T-cell infiltration in tumor tissue lysates were measured by flow cytometry analysis. The gene discussed is CD8A; the disease is neoplasm.