When the same enrichment analysis was performed using only those proteins exhibiting significant differences at the pre-symptomatic stage, the number of differentially regulated pathways was smaller (Figure S3), likely due to the smaller number of proteins used in the analysis, because some of the proteins contributing to cancer-related pathways like Vtn, Thbs1, Flna, Lamb1, and Tln1 did not exhibit significant differences at the pre-symptomatic stage. The gene discussed is VTN; the disease is cancer.