Meanwhile, in individuals with obesity, TNF-α acts on the adipose tissue around blood vessels to promote the activity of oxidases, such as NAD(P)H oxidase, and generate large quantities of ROS, which inactivate nitric oxide (NO) produced by vascular endothelial cells and reduce its relaxing effects [84] on smooth muscle cells leading to vasoconstriction [85]. Here, TNF is linked to obesity due to melanocortin 4 receptor deficiency.