The cells in the recovered solution and migrated cells through PLGA-5/SK and PLGA-10/SK filters showed higher CS marker expression, higher colony-forming ability, and higher CEA secretion than the cells cultivated on TCP plates using colon carcinoma HT-29 cells, which suggested that CSCs (CICs) would be purified from the cells in the recovered solution and migrated cells. Here, CEACAM5 is linked to colon carcinoma.