Therefore, CEA secretion by colon carcinoma HT-29 cells was analyzed in the permeation solution and recovered solution as well as the migrated cells after filtration via the PLGA/SK filters, and the results are shown in Figure 5C, where the CEA generated by the colon carcinoma HT-29 cells was evaluated from the concentration of CEA in the culture medium. This evidence concerns the gene CEACAM5 and colon carcinoma.