Therefore, the expression of CD44 and CD133 on colon carcinoma (HT-29) cells was analyzed using flow cytometry after the permeation of the cells through NM-11 and PLGA/SK filters, where colon carcinoma cells expressing CD133 [37,38,39,40] and CD44 [38,39] are typically considered CSCs (CICs). This evidence concerns the gene CD44 and colon carcinoma.