Our study was designed to investigate the roles of LEF1 in regulating the cell behaviors in colonic adenocarcinoma cells (caco2) using the RNAi method, and analyze the effects of LEF1 expression on the proliferation, motility, and apoptosis of caco2 cells, as well as on the development of the motility relevant microstructures, such as pseudopodia, filopodia, and the polymerization of microfilaments and microtubules. This evidence concerns the gene LEF1 and colon adenocarcinoma.