BRAF and malignant colon neoplasm: Furthermore, the involvement of the ceramide salvage pathway in the mechanisms underlying vemurafenib resistance in BRAF mutant colon cancer cells was also detected by western blot analyses that showed an increased expression of ceramide-producing enzymes CerS2 and CerS6 as well as an up-regulation of ceramide-degrading enzyme ASAH1 in resistant cells under basal conditions and particularly after the exposure to vemurafenib.