THBS2 and pulmonary arterial hypertension: In PAH model mice, the miR-29a-3p level was examined by qPCR and in situ hybridization analyses (Figure 5H,J); THBS2 mRNA and protein in myocardial tissue were measured by qPCR, immunohistochemical staining, and western blotting (Figure 5I,K,L).