We show here that that inhibition of GSL-synthesis with N-[(1R,2R)-1-(2,3-dihy- dro-1,4-benzodioxin-6-yl)-1-hydroxy-3-pyrrolidin-1-ylpropan-2-yl]nonanamide (Genz- 123346 or Genz), n-butyl-deoxynojirimycin (NB-DNJ) (Miglustat) and by targeting UGCG with specific guide RNA by Crispr/Cas9 technology suppressed cell proliferation and tumor spheroid growth in Lovo and HCT116 human colon carcinoma cells in vitro. This evidence concerns the gene UGCG and colon carcinoma.