We next evaluated whether a non-toxic dose of NU7441 (0.04 μM; Figure 4) could enhance the anti-proliferative effects of TORC1 inhibition at sub-therapeutic doses (2.5 to 50 nM sirolimus) on ADPKD cells compared to normal kidney cells. This evidence concerns the gene CRTC1 and autosomal dominant polycystic kidney disease.