In this study, we report on fluorescence europium chelate dyed-nanoparticles (Eu-NP)-based TRF assays to demonstrate the presence of specific proteins and glycans on the surface of EVs in CRC cell culture spend medium and further clinical evaluation of promising candidates in clinical serum samples–We have previously utilized the lectin-NP-based platform successfully to explore the glycosylation of serum glycoproteins CA125 (CA125MGL) and CA15-3 (CA15-3 WGA) in ovarian and breast cancer patients’ samples [31,32]. Here, MUC16 is linked to colorectal carcinoma.