The authors developed a novel method (SarkoSpin) to purify pathological TDP-43 and other aggregated insoluble proteins from frontal cortical brain samples of FTLD-TDP subtypes A (n = 6), B (n = 3), C (n = 6), ALS cases (n = 6), and controls (n = 6) [44]. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.