To mimic patients with higher expression of NEDD4-1 in the clinical setting, we measured the endogenous expression of NEDD4-1 protein in a panel of GBM cell lines using Western blotting and selected the cell lines with the highest expression of NEDD4-1, namely, U251 and U87MG (Figure 2A). The gene discussed is NEDD4; the disease is glioblastoma.