The HCC nodules were strongly associated with the pro-tumorigenic immunosuppressive ICF subclass at 14 weeks (Figure 4a) and, accordingly, the flow cytometry analysis of the intrahepatic cells revealed a lower frequency of CD8+ T cells in tumoral compared to non-tumoral tissue (Figure 4d), while the frequency of Treg per CD4+ T cells was increased (Figure 4e). Here, CD4 is linked to hepatocellular carcinoma.