Moreover, a GSEA comparing tumor versus non-tumor tissues in DEN-treated rats in the 20 weeks group identified a significant enrichment in gene sets related to E2F targets, EMT, G2M checkpoint, Myc targets and KRAS signaling (Figure S3a), while the most significantly depleted gene sets were related to bile acid metabolism, xenobiotic metabolism, fatty acid metabolism, peroxisome and oxidative phosphorylation (Figure S3b). This evidence concerns the gene KRAS and neoplasm.