In the presence of 0.5 μM DJ4 treatment, AML patient samples 1265 (IC50 = 0.50 μM) and 990 (IC50 = 0.26 μM) exhibited a considerable reduction of ~50% in the number of colonies relative to the untreated cells (Figure 2A). This evidence concerns the gene DNAJB6 and acute myeloid leukemia.