Finally, Lowery et al, through the expression profiling analysis of 453 miRNAs from formalin-fixed paraffin-embedded samples of 29 early BC patients, using an artificial neural network (ANN) and RQ-PCR for validation, reported a signature of five miRNAs (miR-520d, miR-181c, miR-302c, miR-376b, and miR-30e-3p), which seemed to accurately predict HER2 status in early BC patients [51]. This evidence concerns the gene ERBB2 and breast cancer.