The presence of this stromal cell population greatly enhances the physiological relevance of our model, as previous studies have outlined the recruitment and induced differentiation of MSCs to CAFs through Notch/akt signaling at the tumour site in osteosarcoma [8], and the metabolic reprogramming of osteosarcoma by CAFs acting as tumour-feeding cells [10]. This evidence concerns the gene TBX1 and osteosarcoma.