We also measured the levels of epithelial–mesenchymal transition (EMT) factors (Snail, Slug, and Twist), epithelial (E-cadherin) markers, and mesenchymal (N-cadherin) markers in tumor tissues since these proteins are known to play important roles in acquiring the invasive and metastatic property of cancer cells [35]. This evidence concerns the gene TWIST1 and neoplasm.