Western blot analysis of ovarian cancer cells cultured on collagen extract or decellularized matrices in the presence or absence of COL11A1 demonstrated that COL11A1 increased phosphorylation of both the serine 78 and 82 sites on HSP27 across ES2 and A2780 (a cisplatin-sensitive ovarian cancer cell line that also expresses low endogenous COL11A1 [9]) in decellularized matrix-based experiments (Figure 1C,D) as well as in ES2 and SKOV3 (an ovarian cancer cell line) in COL11A1 extract experiments (Figure S1G,H). This evidence concerns the gene HSPB1 and ovarian cancer.