To explore whether increasing the levels of ZRF1 enhanced neuroblastoma aggressiveness, we transduced the SK-N-BE(2) and SK-N-AS neuroblastoma cell lines with a ZRF1 lentiviral overexpression vector (Figure 3b), and then analyzed the effects of ZRF1 on different oncogenic properties. The gene discussed is DNAJC2; the disease is neuroblastoma.