In spite of the fact that methylation occurs in any normal breast samples, breast cancer cell lines (HS578T, 21PT, MCF7, T47D) and breast cancer samples exhibited elevated VDR promoter region methylation and significant hypermethylation of CpGs (40–65% dinucleotides), as compared to immortalized normal breast line (HBL100) or normal breast samples. This evidence concerns the gene VDR and breast cancer.