The expression of Hedgehog signaling members has been analyzed in a cohort of 50 human CCA tissues, where GLI1 and PTCH1 have been found overexpressed in approximately 50% and 30% of cases, respectively, together with SHH, detecting a significant activation of Hedgehog in almost 50% of cases [56]. This evidence concerns the gene GLI1 and cholangiocarcinoma.