In a group of 200 patients with intrahepatic cholangiocarcinoma, SHH, IHH, PTCH1, GLI1, GLI2, and SMO have been observed overexpressed in more than 65% of clinical iCCA specimen, compared to non-malignant biliary epithelium, with SHH, SMO, and GLI2 being the most frequently upregulated HH members (>80%). This evidence concerns the gene SMO and intrahepatic cholangiocarcinoma.