GLI1 knockdown significantly reduced the expression of mesenchymal (Vimentin and SNAI1) markers, invasiveness, and the sensitized 5-FU resistant CRC LoVo cell line to 5-FU treatment [80], suggesting EMT regulation to be the major function of GLI1 in mediating 5-FU resistance in CRC. This evidence concerns the gene GLI1 and colorectal carcinoma.