Moreover, in the validation of ANO1, TMEM156, TMEM173 based on GEO datasets from the study presented by Wichmann et al. [25], we indicated that the expression level of ANO1 is the lowest in “atypical IR 1” and the highest in “mesenchymal 3” cluster of HNSCC. This evidence concerns the gene ANO1 and head and neck squamous cell carcinoma.