In order to establish whether intrinsic differences in iNKT cell gene expression could account for IFN-γ/IL-4 cytokine skewing in obese adolescents, iNKT cells from a subset of obese adolescents and age-and sex matched healthy ASD controls were isolated for low-input RNA sequencing (CEL-seq). The gene discussed is IFNG; the disease is atrial septal defect.