Notably, subjects with NAS ≥5, which defines definite NASH (n = 24), had a 1.8 ± 0.2-fold increase in the TAOK3 expression compared with subjects with NAS ≤4, which indicates simple steatosis or borderline NASH (n = 38; P = 0.008). This evidence concerns the gene TAOK3 and metabolic dysfunction-associated steatohepatitis.