Among these m5C RNA methylation genes, we observed the significantly upregulated expression level of NOP2, NSUN2, NSUN5, DNMT3B, and TET2 in the renal cancer cell lines (786-O, Caki-1) compared with human renal tubular epithelial immortalized cell line (HK-2) (∗∗p < 0.01), while NSUN4 was downregulated in renal cell lines. The gene discussed is DNMT3B; the disease is renal carcinoma.