To further evaluate the functional consequences of the expression variability of METTL3 and identify specific subpopulations that are phenotypically relevant to tumor progression, we used a soft three dimensional (3D) fibrin gel culture system to generate stem cell-like tumor-repopulating cells (TRCs) (Liu et al., 2012; Liu J. et al., 2018). This evidence concerns the gene METTL3 and neoplasm.