Finally, we found APC2 was upregulated at the mRNA and protein levels in colorectal cancer cell lines (SW620 and HCT116) with FOXO4 overexpression (OE) for 48 h (Figures 2D,E), and these similar results were verified by luciferase assay (Figure 2F). The gene discussed is FOXO4; the disease is colorectal cancer.