Effective antigenic loading was achieved by coelectroporation of TriMixDC with full-length mRNA encoding HLA-II proteins and melanoma-associated genes (MAA): MAGE-A3, MAGE-C2, tyrosinase or gp100, (TriMixDC-MEL), which allows presentation of the full spectrum of antigenic peptides and leads to a broader MAA-specific T-cell response. Here, PMEL is linked to melanoma.