Additionally, the dual luciferase reporter assay revealed a significant decrease in luciferase activity in MM cells that were cotransfected with miR-135a-5p and the ATF3-3′UTR luciferase reporter vector compared with the luciferase activity in cells cotransfected with the negative control (mimics ctrl) (Figure 3F). This evidence concerns the gene ATF3 and Miyoshi myopathy.