In order to verify the relationship between estrogen, ERα, and liver IR, we observed the expression of ERα in liver tissue of mice at different reperfusion time points (1, 3, 6, 12, and 24 hours) after ischemic 90 min, and observed the liver injury of IR mice treated with estradiol and estrogen inhibitor clomiphene to observe the liver injury of IR mice. Here, ESR1 is linked to digestive system neoplasm.