Fourthly, we detected the expression of MAPK pathway related proteins by western blotting and found that the ERK/MAPK pathway gene expression is decreased after knockout hnRNPA2B1 in colon cancer SW480 cells, while the p38 and JNK signaling pathway gene expression is unchanged, suggesting a specific relationship between hnRNPA2B1 and the ERK/MAPK pathway. Here, HNRNPA2B1 is linked to malignant colon neoplasm.