Initially, parents completed the international ISAAC questionnaire. Based on the questionnaire two groups of children were formed (with and without symptoms of allergy). All children underwent SPT and sera from both groups were tested for sIgE using microarray-based allergen chip (MeDALL allergen chip). Allergen-specific IgE level of = or > 0.3 ISU was considered positive. This evidence concerns the gene IGHE and allergic disease.