Hence, we carried out colocalization studies of mycobacteria with LAMP2 at indicated chase time points after infection with non-pathogenic M. smeg or pathogenic M. tb. Representative images as shown in Supplementary Figure 1D show that lysosomes are located close to mycobacteria-containing phagosomes at 0-h time point for M. smeg infected BMDM, and at 3 h phagosome-lysosome fusion has occurred in these cells. This evidence concerns the gene LAMP2 and infection.