In a rat model of liver fibrosis induced by carbon tetrachloride, mRNA expression of TGF-β1, and genes involved in its downstream signaling, including snail family transcriptional repressor 2 (SLUG), snail family transcriptional repressor (SNAIL), twist-related protein 1 (TWIST1), zinc finger E-box binding homeobox (ZEB)1 and ZEB2, and intrahepatic infiltration of Gr1hi monocytes were profoundly decreased in emodin-treated group (10–40 mg/kg) [75, 76]. The gene discussed is TWIST1; the disease is Hepatic fibrosis.