In TP53 WT human HCC cell lines in vitro, we show that CYP2A6, the human orthologue to murine Cyp2a5, is similarly engaged in a p53-dependent manner in response to CCl4 or Nutlin treatment, as well as in response to treatment with a ROS-inducing agent, cumene hydroperoxide. This evidence concerns the gene TP53 and hepatocellular carcinoma.