After pathway enrichment of 12 key targets, 37 components and 34 pathways were found, among which the top 10 pathways were Bladder cancer, VEGF signaling pathway, Rap1 signaling pathway, Estrogen signaling pathway, HIF-1 signaling pathway, Oxytocin signaling pathway, PI3K-AKT signaling pathway, Pancreatic cancer, Proteoglycans in cancer, and Prolactin signaling pathway. The gene discussed is PRL; the disease is urinary bladder cancer.