The top 10 pathways were shown in Fig. 6A, including Bladder cancer, VEGF signaling pathway, Rap1 signaling pathway, Estrogen signaling pathway, HIF-1 signaling pathway, Oxytocin signaling pathway, PI3K-AKT signaling pathway, Pancreatic cancer, Proteoglycans in cancer, and Prolactin signaling pathway. The gene discussed is VEGFA; the disease is urinary bladder cancer.